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cd36 overexpression adenovirus  (Genechem)

 
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    Genechem cd36 overexpression adenovirus
    Primer sequences.
    Cd36 Overexpression Adenovirus, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36+overexpression+adenovirus/lv+cd36+rnai/pmc10987761-83-1-7
    Average 90 stars, based on 1 article reviews
    cd36 overexpression adenovirus - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Astragaloside IV attenuates renal tubule injury in DKD rats via suppression of CD36-mediated NLRP3 inflammasome activation"

    Article Title: Astragaloside IV attenuates renal tubule injury in DKD rats via suppression of CD36-mediated NLRP3 inflammasome activation

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2024.1285797

    Primer sequences.
    Figure Legend Snippet: Primer sequences.

    Techniques Used: Sequencing

    AS-IV inhibits CD36 production and NLRP3 inflammasome activation in DKD rats. (A) Western blot analysis of ASC, Cleaved-Caspase-1, NLRP3, Cleaved-IL-1β, CD36, GSDMD-D, and Cleaved-GSDMD-D. (B–H) Protein levels of ASC, Cleaved-Caspase-1, NLRP3, Cleaved-IL-1β, and CD36 after normalization to α-tubulin, protein levels of GSDMD-D, and Cleaved-GSDMD-D after normalization to GAPDH. All data are presented as the mean ± SD ( n = 6). ## p < 0.01 vs. Control; * p < 0.05 and ** p < 0.01 vs. Model.
    Figure Legend Snippet: AS-IV inhibits CD36 production and NLRP3 inflammasome activation in DKD rats. (A) Western blot analysis of ASC, Cleaved-Caspase-1, NLRP3, Cleaved-IL-1β, CD36, GSDMD-D, and Cleaved-GSDMD-D. (B–H) Protein levels of ASC, Cleaved-Caspase-1, NLRP3, Cleaved-IL-1β, and CD36 after normalization to α-tubulin, protein levels of GSDMD-D, and Cleaved-GSDMD-D after normalization to GAPDH. All data are presented as the mean ± SD ( n = 6). ## p < 0.01 vs. Control; * p < 0.05 and ** p < 0.01 vs. Model.

    Techniques Used: Activation Assay, Western Blot

    AS-IV inhibits CD36 expression, lipid accumulation, and lipid ROS production in PA-induced HK-2 cells. (B–D) The mRNA expression levels of CD36, CPT1, and PPAP-α were determined by RT-qPCR. (E,F) The protein levels of CD36 were determined by western blot and normalized to α-tubulin. (G,H) Lipid ROS production was detected by flow cytometric analysis. (I) Lipid accumulation in each group was assessed by Oil Red O (ORO) staining under light microscopy (bar = 40 μm). (J) Densitometric analysis of ORO staining. Data are expressed as the mean ± SD. ## p < 0.01 vs. Control; * p < 0.05 and ** p < 0.01 vs. PA.
    Figure Legend Snippet: AS-IV inhibits CD36 expression, lipid accumulation, and lipid ROS production in PA-induced HK-2 cells. (B–D) The mRNA expression levels of CD36, CPT1, and PPAP-α were determined by RT-qPCR. (E,F) The protein levels of CD36 were determined by western blot and normalized to α-tubulin. (G,H) Lipid ROS production was detected by flow cytometric analysis. (I) Lipid accumulation in each group was assessed by Oil Red O (ORO) staining under light microscopy (bar = 40 μm). (J) Densitometric analysis of ORO staining. Data are expressed as the mean ± SD. ## p < 0.01 vs. Control; * p < 0.05 and ** p < 0.01 vs. PA.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Staining, Light Microscopy

    Overexpression of CD36 promotes PA-induced lipid accumulation, lipid ROS production, and activation of the NLRP3 inflammatory body and reverses the protective effect of AS-IV on the expression of lipid peroxidation-related proteins and the NLRP3 inflammatory body in PA-induced HK-2 cells. (A–G) The mRNA expression levels of PPAP-α, CPT1, CD36, ASC, Caspase-1, NLRP3, and IL-1β were determined by RT-qPCR. (H–R) Western blot analysis was conducted to determine the expression of CD36, ASC, Cleaved-Caspase-1, NLRP3, Cleaved-IL-1β, GSDMD-D, Cleaved-GSDMD-D, NGAL, KIM-1 and L-FABP. (S) Lipid ROS production was detected by flow cytometric analysis. (T) Lipid accumulation was assessed by ORO staining under light microscopy (bar = 40 μm). Data are expressed as the mean ± SD. ## p < 0.01 vs. Control; * p < 0.05 and ** p < 0.01 vs. PA; △ p < 0.05 vs. PA + AS-IV 80.
    Figure Legend Snippet: Overexpression of CD36 promotes PA-induced lipid accumulation, lipid ROS production, and activation of the NLRP3 inflammatory body and reverses the protective effect of AS-IV on the expression of lipid peroxidation-related proteins and the NLRP3 inflammatory body in PA-induced HK-2 cells. (A–G) The mRNA expression levels of PPAP-α, CPT1, CD36, ASC, Caspase-1, NLRP3, and IL-1β were determined by RT-qPCR. (H–R) Western blot analysis was conducted to determine the expression of CD36, ASC, Cleaved-Caspase-1, NLRP3, Cleaved-IL-1β, GSDMD-D, Cleaved-GSDMD-D, NGAL, KIM-1 and L-FABP. (S) Lipid ROS production was detected by flow cytometric analysis. (T) Lipid accumulation was assessed by ORO staining under light microscopy (bar = 40 μm). Data are expressed as the mean ± SD. ## p < 0.01 vs. Control; * p < 0.05 and ** p < 0.01 vs. PA; △ p < 0.05 vs. PA + AS-IV 80.

    Techniques Used: Over Expression, Activation Assay, Expressing, Quantitative RT-PCR, Western Blot, Staining, Light Microscopy

    Related Articles

    Over Expression:

    Article Title: Astragaloside IV attenuates renal tubule injury in DKD rats via suppression of CD36-mediated NLRP3 inflammasome activation
    Article Snippet: .. The CD36 overexpression adenovirus was acquired from Genechem Co., Ltd. (Shanghai, China). ..



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    Image Search Results


    Primer sequences.

    Journal: Frontiers in Pharmacology

    Article Title: Astragaloside IV attenuates renal tubule injury in DKD rats via suppression of CD36-mediated NLRP3 inflammasome activation

    doi: 10.3389/fphar.2024.1285797

    Figure Lengend Snippet: Primer sequences.

    Article Snippet: The CD36 overexpression adenovirus was acquired from Genechem Co., Ltd. (Shanghai, China).

    Techniques: Sequencing

    AS-IV inhibits CD36 production and NLRP3 inflammasome activation in DKD rats. (A) Western blot analysis of ASC, Cleaved-Caspase-1, NLRP3, Cleaved-IL-1β, CD36, GSDMD-D, and Cleaved-GSDMD-D. (B–H) Protein levels of ASC, Cleaved-Caspase-1, NLRP3, Cleaved-IL-1β, and CD36 after normalization to α-tubulin, protein levels of GSDMD-D, and Cleaved-GSDMD-D after normalization to GAPDH. All data are presented as the mean ± SD ( n = 6). ## p < 0.01 vs. Control; * p < 0.05 and ** p < 0.01 vs. Model.

    Journal: Frontiers in Pharmacology

    Article Title: Astragaloside IV attenuates renal tubule injury in DKD rats via suppression of CD36-mediated NLRP3 inflammasome activation

    doi: 10.3389/fphar.2024.1285797

    Figure Lengend Snippet: AS-IV inhibits CD36 production and NLRP3 inflammasome activation in DKD rats. (A) Western blot analysis of ASC, Cleaved-Caspase-1, NLRP3, Cleaved-IL-1β, CD36, GSDMD-D, and Cleaved-GSDMD-D. (B–H) Protein levels of ASC, Cleaved-Caspase-1, NLRP3, Cleaved-IL-1β, and CD36 after normalization to α-tubulin, protein levels of GSDMD-D, and Cleaved-GSDMD-D after normalization to GAPDH. All data are presented as the mean ± SD ( n = 6). ## p < 0.01 vs. Control; * p < 0.05 and ** p < 0.01 vs. Model.

    Article Snippet: The CD36 overexpression adenovirus was acquired from Genechem Co., Ltd. (Shanghai, China).

    Techniques: Activation Assay, Western Blot

    AS-IV inhibits CD36 expression, lipid accumulation, and lipid ROS production in PA-induced HK-2 cells. (B–D) The mRNA expression levels of CD36, CPT1, and PPAP-α were determined by RT-qPCR. (E,F) The protein levels of CD36 were determined by western blot and normalized to α-tubulin. (G,H) Lipid ROS production was detected by flow cytometric analysis. (I) Lipid accumulation in each group was assessed by Oil Red O (ORO) staining under light microscopy (bar = 40 μm). (J) Densitometric analysis of ORO staining. Data are expressed as the mean ± SD. ## p < 0.01 vs. Control; * p < 0.05 and ** p < 0.01 vs. PA.

    Journal: Frontiers in Pharmacology

    Article Title: Astragaloside IV attenuates renal tubule injury in DKD rats via suppression of CD36-mediated NLRP3 inflammasome activation

    doi: 10.3389/fphar.2024.1285797

    Figure Lengend Snippet: AS-IV inhibits CD36 expression, lipid accumulation, and lipid ROS production in PA-induced HK-2 cells. (B–D) The mRNA expression levels of CD36, CPT1, and PPAP-α were determined by RT-qPCR. (E,F) The protein levels of CD36 were determined by western blot and normalized to α-tubulin. (G,H) Lipid ROS production was detected by flow cytometric analysis. (I) Lipid accumulation in each group was assessed by Oil Red O (ORO) staining under light microscopy (bar = 40 μm). (J) Densitometric analysis of ORO staining. Data are expressed as the mean ± SD. ## p < 0.01 vs. Control; * p < 0.05 and ** p < 0.01 vs. PA.

    Article Snippet: The CD36 overexpression adenovirus was acquired from Genechem Co., Ltd. (Shanghai, China).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Staining, Light Microscopy

    Overexpression of CD36 promotes PA-induced lipid accumulation, lipid ROS production, and activation of the NLRP3 inflammatory body and reverses the protective effect of AS-IV on the expression of lipid peroxidation-related proteins and the NLRP3 inflammatory body in PA-induced HK-2 cells. (A–G) The mRNA expression levels of PPAP-α, CPT1, CD36, ASC, Caspase-1, NLRP3, and IL-1β were determined by RT-qPCR. (H–R) Western blot analysis was conducted to determine the expression of CD36, ASC, Cleaved-Caspase-1, NLRP3, Cleaved-IL-1β, GSDMD-D, Cleaved-GSDMD-D, NGAL, KIM-1 and L-FABP. (S) Lipid ROS production was detected by flow cytometric analysis. (T) Lipid accumulation was assessed by ORO staining under light microscopy (bar = 40 μm). Data are expressed as the mean ± SD. ## p < 0.01 vs. Control; * p < 0.05 and ** p < 0.01 vs. PA; △ p < 0.05 vs. PA + AS-IV 80.

    Journal: Frontiers in Pharmacology

    Article Title: Astragaloside IV attenuates renal tubule injury in DKD rats via suppression of CD36-mediated NLRP3 inflammasome activation

    doi: 10.3389/fphar.2024.1285797

    Figure Lengend Snippet: Overexpression of CD36 promotes PA-induced lipid accumulation, lipid ROS production, and activation of the NLRP3 inflammatory body and reverses the protective effect of AS-IV on the expression of lipid peroxidation-related proteins and the NLRP3 inflammatory body in PA-induced HK-2 cells. (A–G) The mRNA expression levels of PPAP-α, CPT1, CD36, ASC, Caspase-1, NLRP3, and IL-1β were determined by RT-qPCR. (H–R) Western blot analysis was conducted to determine the expression of CD36, ASC, Cleaved-Caspase-1, NLRP3, Cleaved-IL-1β, GSDMD-D, Cleaved-GSDMD-D, NGAL, KIM-1 and L-FABP. (S) Lipid ROS production was detected by flow cytometric analysis. (T) Lipid accumulation was assessed by ORO staining under light microscopy (bar = 40 μm). Data are expressed as the mean ± SD. ## p < 0.01 vs. Control; * p < 0.05 and ** p < 0.01 vs. PA; △ p < 0.05 vs. PA + AS-IV 80.

    Article Snippet: The CD36 overexpression adenovirus was acquired from Genechem Co., Ltd. (Shanghai, China).

    Techniques: Over Expression, Activation Assay, Expressing, Quantitative RT-PCR, Western Blot, Staining, Light Microscopy